What is a phosphate buffer and why is it important?

A phosphate buffer is a chemical solution that resists changes in pH when small amounts of acid or base are added. This specific phosphate buffer at pH 7.0 is crucial in many biological and biochemical applications because it maintains a neutral environment, which is essential for the stability of proteins and enzymes. For example, it is extensively used in cell culture and laboratory experiments where precise pH is necessary to ensure optimal reactions and results.

How is the phosphate buffer used in laboratory experiments?

In laboratory settings, a phosphate buffer can be used to maintain a stable pH during reactions, which is particularly important in enzymatic assays and biochemical studies. It ensures that the active sites of enzymes remain functional, thus providing consistent results. Researchers often use it to prepare samples or dilutions, effectively isolating the variable they are studying while keeping other factors constant.

Can the phosphate buffer affect the stability of proteins?

Yes, the phosphate buffer plays a significant role in maintaining protein stability. Proteins can denature or lose their functional conformation if the pH shifts significantly outside their optimal range. By using a phosphate buffer at pH 7.0, researchers can provide an environment that minimizes such risks, ensuring that proteins remain stable and functional for accurate experimental outcomes.

What applications can benefit from using a phosphate buffer?

Phosphate buffers are widely utilized in various applications such as DNA extraction, protein purification, and microbiological studies. They are particularly beneficial in cell culture environments where maintaining a specific pH is critical for cell growth and function. Additionally, they facilitate the assessment of drug interactions and behavior in biological testing, making them indispensable in research laboratories.

How should the phosphate buffer be stored for optimal use?

For optimal use, a phosphate buffer should be stored in a tightly sealed container to prevent contamination and evaporation. It is best kept in a cool, dark place to avoid degradation. Additionally, checking the buffer for any changes in appearance or pH before use is recommended to ensure its effectiveness. Proper storage techniques help maintain the solution’s integrity for both short and long-term use in experiments.

Is this phosphate buffer suitable for cell culture applications?

Yes, this phosphate buffer at pH 7.0 [0.2 M] is highly suitable for cell culture applications. Its neutral pH closely mimics physiological conditions, making it ideal for supporting the growth and maintenance of cells in vitro. By maintaining a stable pH, it helps in the delivery of nutrients and the optimal function of cell signaling pathways during experiments.

What is the significance of using a 0.2 M concentration for the phosphate buffer?

The 0.2 M concentration of the phosphate buffer offers a balance between sufficient buffering capacity and practicality in laboratory use. This concentration allows for effective pH stabilization without introducing excessive ionic strength, which could interfere with experimental results. For example, it provides enough buffering in various assays, promoting reliable outcomes in biochemical experiments without compromising the integrity of the samples.

Can the phosphate buffer be used for DNA amplification techniques?

Absolutely! The phosphate buffer is often employed during DNA amplification techniques like PCR (Polymerase Chain Reaction) to maintain an optimal pH environment. This is vital since the quality of the amplified DNA is highly dependent on the pH stability maintained during the procedure. By using a phosphate buffer, researchers can ensure that the amplification process occurs efficiently and with minimal errors.

What should I take into account when preparing this buffer solution?

When preparing a phosphate buffer solution, it is important to carefully calculate the required amounts of the phosphoric acid and its salts to achieve the desired concentration and pH. Additionally, using distilled water to avoid impurities is recommended. Mixing the components thoroughly and adjusting pH with sodium hydroxide or hydrochloric acid as necessary ensures that the solution reaches the target pH of 7.0, crucial for its intended applications.

Where can I buy Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml?

You can buy Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml exclusively on Ubuy, which provides a reliable platform for sourcing high-quality laboratory products. Their extensive catalog and user-friendly interface make it easy to find chemical reagents suited for various applications.

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Is Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml Available to Shop Online in Thailand?

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eCon Lab Supply

Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml

We offer a comprehensive range of biological buffers for use in research, diagnostics, drug development, and manufacturing.

eCon Lab Supply

Item #: 145284083

Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml

Item #: 145284083

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What Stands Out

Precise pH 7.0

Formulated to maintain a stable pH of 7.0, providing reliable buffering capacity for various laboratory applications.

High Concentration

At 0.2 M concentration, this buffer offers enhanced effectiveness in biological and biochemical experiments, ensuring optimal conditions for reactions.

Generous Volume

Available in a convenient 500 ml size, this phosphate buffer meets the demands of extensive lab work without the need for frequent replenishment.

Product Details

Container Type	Bottle
Item Form	Liquid
Item Weight	8 ounces (226.8 grams)
Volume	500 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.85
Brand	eCon Lab Supply
Item Volume	500 ml
Package Information	Bottle

Who Should Buy?

Laboratory Researchers
Ideal for researchers conducting biochemical or molecular biology experiments requiring precise pH control in reactions.
Cell Culture Work
Beneficial for maintaining a stable environment for cell cultures, ensuring optimal growth conditions at physiological pH.
Education Purposes
Useful in educational settings for teaching students about buffer systems and their applications in biological experiments.

Non-scientific Use
Not suitable for everyday applications outside scientific contexts, such as household use or non-laboratory settings.

Product Description

Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml

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Question: How to Shop Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml Online From Ubuy?

Answer: It’s easy to shop Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml Available to Shop Online in Thailand?

Answer: Yes, at Ubuy Thailand this product is available for you to shop at a reasonable price. The Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Comprehensive range of biological buffers.
Ideal for research, diagnostics, and drug development.
Manufactured in ISO 9001 and 13485 certified facility in the USA.
Each lot undergoes stringent Good Manufacturing Practices.
Rigorously tested under certified clean room conditions.
Phosphate buffer ideal for immunochemical staining and preserving living tissues.

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Phosphate Buffer, pH 7.0 [0.2 M] - 500 ml

THB 3720

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